SERUM ENZYMATIC CHANGES AFTER A 50-KM RUNNING IN SUBJECTS WITH DIFFERENT TRAINING LEVEL. A CASE STUDY

Resumo

Running usually induces signs of muscle fibre damage expressed by several biomarkers. However, long lasting running biochemical related studies are scarce. The present case study aimed at evaluating muscle and hepatic enzymatic markers after a 50-km running event in a particular group of military participants.

Based on individual training expertize, four participants were assigned in two groups: HT – 2 highly trained subjects with several years of running training and competition and LT – 2 low trained subjects with only few months of systematic running training.

Venous blood samples were drawn for analysis of serum creatine kinase (CK), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and Aldolase (ALD) before, and immediately after the race and 24, 48 and 72 hours after the race.

Statistical analysis included the percentage of change between baseline and after trial.

All the enzymes increased immediately after trail for both groups with LT showing greater increases for CK and AST. One day after running, CK peaked for both groups with expressive differences between groups. ALD and ALT peaked after one day for HT. For LT, ALD peaked after two days and ALT peaked after three days. AST peaked one day after for both groups but expressively higher for LT. Three days after exertion all values tended to decrease but remained marked higher for LT.

The present study demonstrated that serum enzymes related to muscle and liver damage were increased after a 50-km run. While CK was expressively higher immediately after running and peak one day after, ALD, AST, and ALT showed different peaks. Participants’ training level seems being a main determinant for enzymatic changes after prolonged running and during recovery.